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COMBINED TREATMENT OF THE INFANTILE BRONCHIAL ASTHMA

COMBINED TREATMENT OF THE INFANTILE BRONCHIAL ASTHMA

A.A. Lebedenko, A.V. Tarakanov,
N.P. Milutina
Rostov-on-Don

It is well known, that a chronic allergic inflammation of bronchus membrane accompanied by their hyperreactivity and revealing itself clinically by repeated asphyxiation attacks lies in the basis of bronchial asthma. Using broncholytic drugs in the exacerbation period lets us relieve patients’ state, but stabilizing the status often demands a longer period of time. The fact, that a lot of patients have drug allergy restricting the scope of drugs used, is also very important. Prescribing broncholytics also doesn’t influence the most important pathogenetic aspects of the disease and, particularly, the processes of lipid peroxidation (LP) accompanied by accumulating abundant number of free radicals facilitating progressing bronchus membrane injury.

In this connection using physical factors of influencing central and local regulation of bronchopulmonary homeostasis, and particularly SCENAR therapy, is of great interest. SCENAR therapy is directed to the different hierarchical body levels for achieving a more evident clinical result.

The following aspects should be taken into account. There are the accepted standards of treating bronchial asthma. The insuring companies pay for the treatment proceeding from these standards. So, the treatment in state hospitals is restricted with certain limits. On the first stage of treatment, which is represented in this work, the treatment was combined.

The aim of the work is increasing the effectiveness of treatment and, particularly, improving the immediate results in the exacerbation of asthma, activating antioxidant protection, improving the distant prognosis for a disease. The problem put by is solved by carrying out an individually dosed influence of skin coverlets, which are topographically connected with bronchopulmonary system during the exacerbation period (SCENAR-97.4). The frequency was 90 Hz, general influence duration – 30-40 minutes, the course duration – 10-14 days. First 3 paths are influenced, then – adjacent regions (humeroscapular, posterior neck surface). The session is finished with influencing the sternum and jugular vein.

Before, in the process and after the course of treatment the dynamics of body reactions is taken into consideration: realizing a questioning status evaluation, investigating external respiration function determining expiration peak speed (EPS) with Peak Flow Meter, investigating LP parameters and body antioxidant systems. 12 patients with bronchial asthma were treated, taking into account their age and severity of the disease.

Let us give some examples of the concrete treatment including SCENAR therapy:
1. K.D., 13 years old, c.h. ? 421, diagnosis: bronchial asthma, atopic form, severe course, exacerbation phase. On admission there was dyspnea, cough, difficult expectoration discharge, EPS decrease up to 200 l/min (N – 350 l/min). SENAR therapy was done simultaneously with broncholytic therapy (berodual inhalations) according to the method described above for 40 minutes daily, during 14 days. Immediate treatment results: dyspnea was arrested on the first day, EPS indices were normalized before day 4, state of health and tolerance to physical activity improved, the balance of oxidative and antioxidative systems stabilized (diene conjugate, Shiff’s bases, malonic dialdehyde, superoxide dismutase). Distant treatment results: we managed to decrease inhalation corticosteroids’ dose up to 250 mcg per day. There were no exacerbations of the disease.
2. G.M., 11 years old, c.h. ? 2809, diagnosis: bronchial asthma, atopic form, severe course, hormone-dependent, exacerbation phase. On admission the state was severe, there was asphyxiation, no expectoration discharge, expressed anxiety, uniformly diminished breath sounds in the lungs by auscultation, EPS decrease by 3 times if compared with the norm. SCENAR therapy was carried out according to the above-described method daily (40 minutes each day, during 14 days) together with intravenous introduction of glucose-saline solution, eufillin, prednizolon, berotek inhalations. On the second day the patient’s state considerably improved, dyspnea decreased, expectoration began to discharge, physical pattern in the lungs improved. EPS began increasing since day 3, there was no necessity for parenteral introduction of drugs, tolerance to physical activity improved.
In 12 days EPS level reached 75% of the normal, which was the best result of the last year, the indices of the body antioxidative system improved. Distant treatment results: we managed to decrease corticosteroids’ dose by 30%.
3. K.D., 9 years old, c.h. ? 1106, diagnosis: bronchial asthma, atopic form, severe course, exacerbation phase. On admission the state was severe, but the patient did without oxygen. He coughed with non-secreting expectoration, dyspnea, abundant sibilant rales in the lungs, EPS decrease up to 120 l/min (N – 310 l/min). Besides berodual inhalations via nebuliser and one-fold eufillin injection, the patient underwent SCENAR therapy daily (40 minutes each day, during 10 days). Dyspnea was arrested on the first day. EPS increased up to 90 l/min on the second day, by the 8th day peak exhale rate made 86% of the norm, on the 14th day there was progress in the antioxidative body system. Immediate treatment results: rapid relief of dyspnea, normalization of general status. Follow-up treatment results: inhalation corticosteroids’ dose was decreased from 1000 to 500 mcg.
4. N.D., 13 years old, c.h. ? 969, diagnosis: bronchial asthma, atopic form, medium-severe course, exacerbation phase. On admission the patient complained of dyspnea, restraint in the chest, inefficient cough. EPS was 68% of the norm. Besides berotek inhalations via nebuliser and expectorants, the patient underwent SCENAR therapy (30 minutes each day, during 10 days). Dyspnea was arrested on the first day, since the second day expectoration secreted better, physical weight was born more tolerably. On the third day EPS reached 100% level (390 l/min). There was no need in parenteral introduction of drugs. Indices of the antioxidative system considerably improved on the 10th day. Immediate treatment results: rapid status stabilization, arresting dyspnea. Follow-up treatment results: no disease exacerbations during 6 months.

Let us give some examples of treatment in the control group without using SCENAR therapy.
1. Ch.D., 12 years old, c.h. ? 1013, diagnosis: bronchial asthma, atopic form, medium-severe course, exacerbation phase. On admission the patient complained of inefficient cough and dyspnea. EPS was 67% of the norm. Dyspnea was arrested on the third day against a background of broncholytic therapy, but on the 8th day of the patient’s stay in the in-patient department there was an asphyxiation attack. There was a full expectoration only since the 4th day. We did not manage to achieve a considerable EPS increase during 14 days. There were no considerable changes in the indices of antioxidative body system.
2. P.D., 9 years old, c.h. ? 665, diagnosis: bronchial asthma, atopic form, medium-severe course, exacerbation phase. Dyspnea was arrested on the third day against a background of broncholytic therapy, but there was iterated disease exacerbation after abolishing the therapy. On admission EPS was 56% of the norm, and we had managed to increase it up to 76% only by the 9th day of the therapy. There was poor physical load tolerance.
3. K.Y., 10 years old, c.h. ? 1255, diagnosis: bronchial asthma, atopic form, medium-severe course, exacerbation phase. The state stabilized on the 4th day against a background of broncholytic therapy, but we did not manage to achieve a considerable EPS increase during the time of a child’s stay in the in-patient department. The indices of oxidative and antioxidative body systems did not change considerably during 14 days.

Some treatment results are given in the Table 1.

Let us also point out economic effectiveness of the combined treatment of bronchial asthma: minor amount of drugs and, mainly for parenteral injection; decreasing the dose of drugs taken in the remission period and mainly glucocorticosteroids.

The suggested approach may be used in the specialized allergic departments as well as in hospitals of general profile. Compact and reliable SCENAR device gives an opportunity to work with big groups of patients.

Treatment results Control SCENAR
Arresting exacerbation 78% 94% *
The necessity of additional measures for arresting exacerbations 22% 6% *
Disappearance of respiratory failure on the first day 54% 78% *
Normalizing peak exhale rate during 3 days 66% 84% *
Normalizing peak exhale rate by the end of treatment 84% 94% *

 

Plasma’s catalase, nmole ?202/ml 24,11±1,32 26,97±1,11
Erythrocytes’ catalase, nmole ?202/mgHb 20,35±1,04 24,60±1,13 *
Schiff bases (plasma), rel.un. 1,52±0,14 1,85±0,13
Diene conjugates (plasma), nmole/ml 9,88±0,81 11,91±0,53 *
Decreasing the dose of oral and inhalation corticosteroids 6% 54% *
Rare disesase exacerbations 6% 54% *

Note: * – difference validity by ? < 0,05.

CONCLUSION
1. Additional SCENAR therapy in the conditions of in-patient department lets us improve immediate treatment results considerably. Rapid dyspnea arresting, early expectoration and rapid increase of peak exhale rate testify to this fact.
2. The necessity of parenteral drug injections decreases, and it has evident advantages, while psychic traumatic load upon a child decreases.
3. If compared to the control group, there is a rapid improvement of patients’ general status, increase of their physical activity and consequently the quality of life.
4. Follow-up treatment results improve (the frequency of disease exacerbations lowers, the dose of inhalation and oral glucocorticosteroids taken in the interictal period of the disease).
5. Antioxidative body system is activated, and it prevents the disease from progressing pathogenetically.

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RITM Australia and RITM SCENAR Institute congratulate RITM OKB ZAO for 30 years of development in the technology  that gives the world the ultimate gift – the enhanced ability for the human body to heal itself.

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Antioxidant and membrane protecting effects of SCENAR-therapy in treating opium addiction

Authors:           M.V. Ovsyannikov. A.V. Tarakanov, N.P. Milyutina, Ya.Z. Grinberg, S.L. Maslovskiy, G.A. Tarakanova

                          Emergency Department, Rostov State Medical University, Department of Biochemistry and Microbiology, Rostov State University, City Psychoneurologic Dispensary (Rostov-on-Don), Clinical Trials Department, RITM OKB ZAO (Taganrog)

 

Article name:   Antioxidant and membrane protecting effects of SCENAR-therapy in treating opium addiction

Summary:

The research showed that including SCENAR-therapy into the complex treatment of opium-addicted patients greatly increases the produced therapeutic effect and decreases the intensity of oxidative stress. Such an effect is achieved by stimulating antioxidative enzymes – superoxide dismutase and catalase in erythrocytes – and inhibiting the intensity of free-radical processes in the blood. Decreasing the level of lipid peroxidation in erythrocyte membranes provides increase in stability and normalization of erythrocyte membrane structure. 

Key words: opium-addiction, SCENAR-therapy, free-radical processes, antioxidant enzymes, erythrocyte membrane structure, oxidative stress.

Oxidative stress caused by abrupt change in redox-condition of the body and activation of free-radical oxidation [8] plays an important role in molecular mechanisms of opium addiction.  In turn, damaging effects of the oxidative stress contribute to dysmorphology in biomembranes; that intensifies the imbalance in harmonic-mediator exchange and causes the development of concurrent somatic pathologies in drug addiction [9, 13]. Recent researches pay much attention to different methods of energy-information influence (SCENAR-therapy, laser therapy, acupuncture, etc). It was found out that these methods are often more effective than pharmacological correction of metabolism imbalance [3, 12]. Today it is known that complex therapy for opium addiction at a stage of abstinence relief and next treatment stages, as well as arresting endogenous intoxication syndrome and correcting the harmonic-mediator status [2], should have evident         anti-oxidant effect to limit damaging effects of the oxidative stress [8,9]. Our previous experimental and clinical   researches show effectiveness of SCENAR-therapy as non-drug therapy for controlling free-radical oxidation [7, 12]. So, there are certain pathogenetical reasons to include SCENAR-therapy into the complex of therapies for treating          opium-addicted patients, it can also help to reduce total “pharmacological load” thus reducing the exertion of body detoxication systems.

The research was aimed to determine the influence of complex treatment including SCENAR-therapy on the indices of oxidant stress in the blood and structural state of erythrocyte membranes in opium addiction during abstinence relief.

MATERIALS AND METHODS 

56 opium-addicted patients (17-35 years old, disease duration – 1,5-10 years) participated in the clinicolaboratory examination. The patients got to clinic diagnosed with persistent abstinent syndrome and were divided into two groups depending on the therapy used:

1 group – patients undergone conventional treatment

2 group – patients undergone conventional treatment complemented with SCENAR-therapy.

SCENAR-97.4 was the device used in the research. All the sessions were done in the individual dose mode, 30-40 min each session, 10-14 days whole  treatment course. Dynamic of body response was registered before, during and after the treatment course: we asked our patients to fill in the health status questionnaire, estimated redox condition of their body and indices of membrane homeostasis. The control group consisted of 15 almost healthy donors of appropriate sex and age.

Test material was venous blood stabilized by heparin 50 u/ml. Blood samples were centrifuged at 3000 r/min for 15 minutes and plasma was separated. Erythrocyte sediment was washed three times by 10ml of 0,15M NaCl solution in tris-H?l buffer pH 7,4 and then erythrocyte suspension with equal protein content (0,5mg/l) and 1% hemolysate was made. The intensity of free-radical processes was estimated according to H2O2-luminol-induced chemiluminescence parameters [14] and content of lipid peroxidation products. The content of primary lipid peroxidation products – diene conjugates  – was determined according to the method [10], the content of secondary products – malondialdehyde – according to the method [11], end products – schiff bases – according to the method [15]. The state of antioxidant system was estimated by the activity of antioxidant ferments – superoxide scavengers [16] and catalses [4] in erythrocytes. Erythrocyte membrane stability was estimated according to the ectoglobular heumoglobin level of total peroxidase activity in blood plasma [6]. Structural condition of erythrocyte membranes was investigated using the method of piren fluorescent probe lateral diffusion [1]. Microviscosity of lipid bilayer and zones of protein-lipid contacts was determined by the value of piren excimerisation coefficients – F?/F?(334) and F?/F?(282), that equal the correlation of fluorescence of its excimeric  (Fe) and monomeric (Fm) forms at the exciting light wave length 334nm and 282nm. Restructure of membrane proteins was determined by the effectiveness of non-radiating energy transmit from membrane proteins to piren – F0-F/F0. Statistical analysis of the results was done using Student’s t-criterion.

RESEARCH RESULTS AND DISCUSSION

The research shows that in both clinical groups of opium-addicted patients in abstinence condition significant increase in effectiveness of free-radical processes (Table 1,2) in blood plasma and erythrocyte membranes is observed.

Table 1.

Intensity of H2O2-luminol-induced chemiluminescence and lipid peroxidation in blood plasma of opium-addicted patients in the multitherapy during the period of abstinent relief (M±m).

Indices n Treatment period Chemiluminescence indices Lipid peroxidation indices
?

mm

Sm*104

rel.unit

Diene conjugate

nmol/ml

Malonic dialdehyde

nmol/ml

Schiff bases

u.fl/ml

Donors 10-15 40.0±7.0 74.0±13.0 12.38±1.71 20.87±1.58 1.062±0.009
1 group

(conventional

treatment)

15-22 Before 69.5±5.0* 108.0±7.0* 18.24±2.28* 38.98±8.76* 2.053±0.051*
After 92.0±3.0*** 119.0±4.0* 20.99±1.54* 43.49±11.16* 2.149±0.060*
2 group

(conventional

treatment + SCENAR-therapy)

15-20 Before 64.7±6.8* 129.4±8.0* 22.14±2.12* 37.63±2.87* 1.818±0.107*
After 34.0±8.0** 74.9±8.5** 15.83±2.02** 28.61±8.18** 0.920±0.134**

Note: here and in Table 2,3

* – reliability of differences compared to the checkout (donors), p<0.05-0.001;

* * – reliability of differences between indices before and after the treatment p<0.05-0.001

Table 2

Intensity of lipid peroxidation, activity of superoxide scavengers and catalse in erythrocytes of opium-addicted patients in the multitherapy during the period of abstinent relief (M±m).

Indices n Treatment period Diene conjugate

nmol/ml

Malonic dialdehyde

nmol/ml

 

 

Schiff bases

u.fl/ml

 

 

Superoxide scavengers u/mg hb

Catalase

nmol H2O2/mg Hb

Donors

 

10-15 4.34±0.13 3.93±0.31 0.553±0.040 3.55±0.29 27.61±2.78
1 group

(conventional

management)

15-22 Before 7.02±0.31* 8.80±0.32* 0.765±0.068* 2.66±0.13* 19.88±2.12*
After 8.19±0.41*** 10.03±1.0* 0.543±0.059** 3.15±0.36* 19.16±0.63*
2 group

(conventional

management + SCENAR-therapy)

15-20 Before 9.63±1.41* 8.33±0.34* 0.997±0.085* 2.57±0.15* 17.74±2.06*
After 5.47±0.53** 5.57±0.24*** 0.459±0.042** 3.37±0.12** 24.10±2.13**

 

The indices of H2O2-luminal-induced chemiluminescence – quick flash height (m) and light sum – 49-79%  exceeds the norm (donors) before treatment, that signs extra generation of oxygen active forms, which have high cytotoxic potential and can  initiate lipid peroxidation.  Table 1 and 2 show that the content of molecular products – diene conjugate, malonic dialdehyde, schiff bases – in blood plasma and erythrocyte membranes in both groups of patients exceeds the control level by 38-122%.  During the increase of free-radical process intensity in erythrocytes of addicted patients we can observe inhibition of antioxidant ferments of superoxide scavengers and catalse by 25-36%, which control the free-radical process at the stage of oxygen activation and origin of lipid peroxidation chain process. So, we can observe the development of oxidative stress in opium-addicted patients and that has a great number of damaging effects. That can be proved both by our research results [8,9] and by researches made by other authors [13].

Our research showed that in the 1 group of addicted patients that undergone conventional treatment intensity of free-radical processes in the blood remains as high, as it was registered before the treatment. The only exception is that after the treatment the content of lipid peroxidation end products – schiff bases – in erythrocyte membranes decreased by 29% (Table 1,2).

In the 2 group of patients that undergone conventional treatment combined with SCENAR-therapy, intensity of free-radical processes in blood, in a whole, returns to the stationary level typical for the norm. During the treatment chemiluminescence parameters decrease by 42-47% and the content of lipid peroxidation products in blood plasma reduces by 24-49%, in erythrocyte membranes – 35-54% relative to the initial level, except malonic dialdehyde content, which remains 42% higher than the control index even after the treatment (Table 1,2).

In the 1 group of patients, where the treatment didn’t influence the intensity of      free-radical processes in the blood, we can observe the disfunction of conjugated antioxidant enzymes in erythrocytes. Superoxide dismutase activity during the treatment approaches the control indices, while catalase activity is by 31% inhibited compared to the norm. On a contrary, in the 2 patient group, where the treatment helped to normalize most of the chemiluminescence and lipid peroxidation parameters, during the treatment we can observe stimulation of activity of superoxide dismutase and catalase in erythrocytes. Activity increment of antioxidant enzymes in erythrocytes is 31-96% compared to the initial background (Table 2).

So, complementing complex treatment of opium-addicted patients with SCENAR-therapy significantly decreases the manifestation of oxidative stress by activating the most important erythrocyte enzymes and decreasing the intensity of free-radical processes in the blood. So, restoration of redox state in the blood can be considered as one of the most important mechanisms of therapeutic impact of SCENAR-therapy in treating drug addiction.

Uncompensated activation of lipid peroxidation in erythrocyte membranes among patients of the 1 and 2 groups before the treatment causes destabilization and destructuring. Significant level increase of ectoglobular heumoglobin of total peroxidase activity (66-88% from the norm) in blood plasma in both patient groups before the treatment (Table 3) shows that levels of ectoglobular heumoglobin and total peroxidase activity in blood plasma are considered to be sensitive parameters of erythrocyte membrane stability [6], and their increase reflects the increase of plasma prooxidant potential as it contributes to additional activation of lipid peroxidation due to formation of oxygen active forms and hemoglobin       ferril– radical.

Table 3 shows that erythrocyte membranes of drug-addicted patients from two groups observe the 17-21% decrease of piren probe excimerization coefficient Fe/F?(334) and the 27-33% increase of the F?/F? parameter (282). It’s known that degree of piren excimerization is in the inverse dependence on lipid phase microviscosity [1, 5]. The research results show opposite changes in microviscosity of different erythrocyte membrane compartments, that increases in lipidic bilayer and decreases in protein–lipid contact zones presented by annular lipids that form microenvironment of membrane proteins.

Table 3

Ectoglobular heumoglobin level, total peroxidase activity in blood plasma and structural state of erythrocyte membranes among opium-addicted in the multitherapy during the abstinent relief (?±m).

Indices n Treatment period Blood plasma Erythrocyte membranes
Ectoglobular heumoglobin mcM/l Total peroxidase activity un/ml Fe/F?(334) Fe/F?(282) F0-F/F0
Donors 10-15 4.84±0.44 3.56±0.45 0.75±0,05 0.98±0.02 0.152±0.006
1 group

(conventional

treatment)

15-22 before 8.94±0.42* 5.91±1.30* 0.59±0.03* 1.24±0.07* 0.129±0.004*
after 9.38±2.46* 6.51±1.69* 0.62±0.04* 1.11±0.10* 0.135±0.003*
2 group

(conventional

treatment + SCENAR-therapy)

15-20 before 8.51±0.75* 6.70±1.30* 0.62±0.03* 1.30±0.08* 0.128±0.006*
after 4.98±0.60** 3.05±0.26** 0.80±0.05** 1.01±0.11** 0.144±0.006

At the same time it was registered that F0-F/F0 parameter decreased by 15-16% and that indicates the decrease in effectiveness of nonradiating energy transfer of electronic excitation from membrane proteins to piren and indicates destructuring in membrane proteins of erythrocytes. Modification of erythrocyte membrane structure inevitably causes inhibition in functionally important membrane processes, as well as impairment of membrane viscoelastic properties and decrease in hemolytic resistance of erythrocytes.

After the treatment course in the 1 group of patients most of the structure disturbances in the erythrocyte membrane remain the same, except the normalization of annular lipid microviscosity Fe/F?(282) (Table 3).

Conventional treatment combined with SCENAR-therapy in the 2 group of patients contributes to normalization of erythrocyte membrane structural parameters; fluidity of lipid bilayer and annular lipids normalizes, structural changes in membrane proteins eliminate and that improves the structure and function of erythrocyte membranes. It seems that there are different ways to produce membrane stabilizing effect of the SCENAR-therapy:  a) normalize the structure of erythrocytes circulating in blood channel by inhibiting free-radical processes and stimulating antioxidant enzymes; b) change the structure of erythrocyte population to its rejuvenation. It’s known that young erythrocytes are characterized by hyperplasticity and optimal viscoelastic properties [5].

So, complementing complex treatment of opium-addicted patients with             SCENAR-therapy helps to decrease negative manifestations of oxidative stress by decreasing the intensity of free-radical processes, stimulating the antioxidant protection enzymes in blood, increasing stability and restoring structural homeostasis of the erythrocyte membranes.

 

Bibliography:

  1. Yu. A. Vladimirov, G. E. Bobretsov. Fluorescent probes in studying biological membranes. M. Nauka, 1980. – 320p.
  2. A. I. Golovko, L. V. Leontieva, S. I. Golovko and co-authors. Neurochemical principles of instant opiate intoxication // Issues of Medical Chemistry, 2002, v. 48, ?2, p.154-173.
  3. V. G. Zilov, K. V. Sudakov, O. I. Epstein. Elements of informational biology and medicine. M. MSFU, 2000. – 248p.
  4. M. A. Korolyk, L. I. Ivanova, I. G. Maiorova and co-authors. Method for identifying catalase activity // Laboratory, 1988, ? 1, p.16-19.
  5. V.S. Lee, E. M. Halilov, V.I. Saburova and co-authors. Lipid composition and structural functionality of eretrocytes membranes of different age // Issued of Medical Chemistry. 1982, ?6, p.66-71
  6. A. I. Lukash, V. V. Vnukov, A. A. Anayan and co-authors. Metallic compounds of blood plasma in hybaroxia. Rostov-on-Don.  Scientific Medical Centre “Logos”. 1996. 108p.
  7. S. L. Maslovsky, N. P. Milytina, V.V. Vnukov and co-authors. Using SCENAR in correcting rats’ structure-metabolic homeostasis in oxidative stress // Modern Technologies in Science, 2004, ?4, p. 44-45.
  8. M. V. Ovsyannikov, S.L. Maslovsky, N. P. Milytina. Oxidative stress in pathogenesis of opiomania // Proceedings of Institutions of Higher Education in North Caucasian region. Natural science, 2004, ?5 (17), – p.42-46
  9. M. V. Ovsyannikov, S.L. Maslovsky, N. P. Milytina. Membrane structure of eretrocytes in pathogenesis of opiomania // Biological membranes, 2005, v. 22, ?2, p.100-104
  10. I. D. Stalnaya, T. D. Garishvili. Method for identifying diene conjugation of unsaturated highest acids // Modern Biochemical Methods. M, Medicine. 1977. – p.63-64
  11. I. D. Stalnaya, T. D. Garishvili. Method for identifying malondialdehyde with the help of thiobarbituric acid // Modern Biochemical Methods. M, Medicine. 1977. – p.66-68
  12. A. V. Tarakanov, Ya. Z. Grinberg, N. P. Milytina. Universal mechanism of SCENAR-effect in oxidative stress // Reflexotherapy, 2003, ?4 (7), – p.41-45
  13. N. N. Usmanova. Opiomania somatic complications in adolescence and the role of free radical processes in their pathogenesis. Abstract of a thesis, candidate of biological sciences. Moscow, 2000, 20p.
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  15. Bidlack W.R., Tappel A.L. Fluorescent products of phospholipids during lipid peroxidation // Lipids, 1973, V.8, ? 4. – P.203-209.
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SCENAR-THERAPY CLINICAL EFFECTIVENESS FOR THE PATIENTS WITH TUBAL-PERITONEAL INFERTILITY

L.V.Borovkova, I.E.Holmogorova, V.D.Uchaikina, E.V.Chelnokova
Nizhny Novgorod State Medical Academy

N.A.SEMASHKO MPO (MEDICAL PUBLIC OFFICE) REGIONAL CLINICAL HOSPITAL NIZHNY NOVGOROD

SCENAR-THERAPY CLINICAL EFFECTIVENESS FOR THE PATIENTS WITH TUBAL-PERITONEAL INFERTILITY

Background. Tubal-peritoneal factor occupies the first place in the structure of ethiopathogenetic reasons of fertility and it counts in about 40-60% of all female fertility cases [1, 2]. Main reasons of tubal-peritoneal fertility are inflammatory diseases (both of the bacterial and viral genesis) and adhesions formation in the postoperative period. Many researchers note that it is rather difficult to restore fertility in this group of patients even by using endosurgical methods of treatment. Registered rate of pregnancy coming after reconstructive plastic surgeries done using operative laparoscopy doesn’t exceed 21-28% [3, 4]. We can conclude that judging by ways of increasing the effectiveness of tubal infertility treatment and postoperative adhesive process, progress will be determined not only by perfecting the surgical technique itself (which has reached almost 100% effectiveness in eliminating the anatomic fallopian tubes blocking), but more than that, by the postoperative rehabilitation therapy success (which is meant for correcting the impaired tube function with a restored lumen and preventing the postoperative adhesive process).

Chronic inflammatory diseases of the female pelvic organs cause systematic changes in the female body involving not only inner genitals, but almost all homeostasis links. Psycho-emotional sphere, autonomic body regulation, immunological status, gonadotropic and ovarian hormones metabolism and secretion [5,6] become involved into the pathological process. The methods of treatment used appear to be not so successful, which is caused by the persisting principle of the predominant influence upon the organ pathology. The state of central regulation mechanisms (psycho-emotional state, autonomic nervous regulation) and a concomitant extragenital pathology character are not always taken into consideration [1,2].

So, we are looking for the methods of treating the patients with tubal-peritoneal infertility, which would not only eliminate the mechanic fallopian tubes blocking, but would also have optimizing influence upon all homeostasis parameters.

Research objective: To evaluate SCENAR-therapy clinical effectiveness for the patients with infertility of tubal-peritoneal genesis.

Methods of statistical analysis of the material: Statistical data was processed in a Statistica 6.0 program. The regulacy check was done using the Kolmogorov and Smornov’s criterion. Actual data is presented as <average + standard deviation> (?+?). We used Student’s t-criterion for dependent groups to determine dependent samples differences reliability taking a regular distribution law. If the analyzed samples’ distribution differed from the regular one, we used the Wilcoxon criterion for dependent groups. We took a critical level equal to 0.05 in our research.

Data for study and discussing it
We have done a complex examination of 111 women with tubal-peritoneal infertility (aged 29-39, (28.4 + 1.7) on average). Total infertility duration was from 1 to 15 years (8.3 + 2.1 years on average). We traced back that 15% of patients had the II degree adhesive process, 51% – III degree, 34% – IV degree respectively. All examined women were divided into 3 groups: the first group (the control group) included women who had just undergone laparoscopy (30 women), the second group included women who had just undergone laparoscopy and SCENAR-therapy (39 women), and the third group included women who had undergone only SCENAR therapy (42 women) (the infertility factor was revealed on the uteroturbography).

We used SCENAR 97.4+, 97.5 (or SCENAR-1-NT) and SCENAR-DE devices with vaginal probes in the treatment. SCENAR influence was done according to the general rules taking into consideration the clinical picture, combining and interchanging influence modes and methods in IDM and SDM, and also using SCENAR-DE with vaginal probe. The patients from the second group were treated on the second day after the laparascopy. The courses included 10-15 sessions depending on the clinical picture. The patients from the third group underwent treatment in the second phase of the menstrual cycle (10-15 sessions).

Table 1. Restoring the uterine tubes patency and getting pregnant after tubal-peritoneal infertility depending on the method of treatment and infertility duration.

Laparascopy (control)

N=30

Laparascopy +SCENAR

N=39

SCENAR

N=42

Total percentage of restoring the uterine tubes patency 33% (10) 56% (22) 64% (27)
Infertility up to 3 years 40% (4) 60% (6) 83% (10)
Infertility up to 3-5 years 40% (4) 57% (8) 62% (8)
Infertility more than 5 years 20% (2) 53% (8) 53% (9)
Total percentage of pregnancies 23% (7) 51% (20) 45% (19)
Infertility up to 3 years 40% (4) 50% (5) 58% (7)
Infertility up to 3-5 years 20% (2) 57% (8) 54% (7)
Infertility more than 5 years 10% (1) 47% (7) 29% (5)

As we can see in Table 1, the patients from the second group (51%) and from the third group (45%) achieved the best results in restoring the uterine tubes patency and getting pregnant.

We also analyzed the mechanism of SCENAR influence upon the main homeostasis parameters.

We investigated the general antioxidant activity of blood serum and the intensity of free-radical acidification process using software biochemoluminometer BCL-06M. The SCENAR-therapy resulted in lipid peroxidation (LPO) products suppression, which produced antimitotic and cytotoxic influence upon living cells and tissues. We also marked the increasing antioxidative system (AOS) activity, catalase and superoxide dismutase (SOD) ferments and the intoxication decrease (Table 2). Given parameters depended on the fertility restoration methods.

Table 2. AOS and LPO Status Before and After SCENAR-Therapy

Indices Laparascopy (control)

N=30

Laparascopy +SCENAR

N=39

SCENAR

N=42

Before the treatment After the treatment Before the treatment After the treatment Before the treatment After the treatment
Imax (mV) 1.986+0.013 1.945+0.234 1.942+0.272 1.785+1.194 1.965+0.172 1.878+0.136
AOS (rel.un.) 0.046+0.008 0.05+0.001 0.047+0.009 0.055+0.006 0.045+0.007 0.053+0.007
SOD (act.un./g Hb) 93.92+26.14 94.12+21.07 92.55+41.59 134.9+49.12 85.49+25.1 111.39+29.53
Catalase

(act.un./g Hb)

96.31+34.78 101.56+27.32 95.41+24.53 134.32+30.89 95.45+14.68 136.05+28.06
Intoxication (rel.un.) 9.48+1.27 8.93+2.49 9.68+1.25   7.69+2.11 10.31+0.95 7.45+1.87

N o t e. Differences reliability according to the Student’s t-criterion: between the indices before and after the treatment, as well as between the control group and other groups, with ?<0.05.

After finishing the tubal-peritoneal infertility treatment, in the control group, where only laparascopy was used, there is a 2.1% decrease in LPO activity and an 8.7% increase in blood indices. There is an 8.8% LPO activity decrease and 17% AOS indices increase in the second group (laparascopy+SCENAR). There is a 4.6% LPO activity decrease and 17.8% AOS indices increase in the group where only SCENAR was used as a treatment.

Complex examination of the patients with tubal-peritoneal infertility included ultra sound investigation of small pelvis organs with small pelvis vessels Doppler velocimetry. We noticed slight blood flow changes in small pelvis vessels by Doppler velocimetry in the control group: there was only a 1.4% increase of the resistance index in the uterine arteries and a 9.5% increase in the ovarian arteries; laparascopy+SCENAR group – 5.3% and 18.4%; the third group – 5.8% and 18.7% correspondingly.

All patients in different groups underwent autonomic status dynamics investigation (Table 3). 100% of women had an autonomic dysfunction before the treatment.

Table 3. Autonomic Status Dynamics of Patients (Me (interquartile range), where Me is a median line)

Autonomic function (point*) Laparascopy (control)

N=30

Laparascopy +SCENAR

N=39

SCENAR

N=42

Before the treatment 22 (20-31) 25 (20-28) 24 (22-29)
After the treatment 22 (20-31) 7 (6-10) 10 (7-11)

*0-15 points – normal autonomic status, >15 points – dysautonomia.

N o t e. The differences reliability according to Wilcoxon criterion: between the indices before and after SCENAR-therapy with ?<0.05.

Autonomic status in the second and the third groups normalized after SCENAR-therapy. The indices in the first group didn’t change.

 

We also used a cardiointervalography (CIG) method in analyzing the autonomic status. We analyzed CIG parameters and found out that SNS tonus prevailed in all patients, and the function of the parasympathetic part decreased. Regulatory systems tension index also deviated from the norm and was considerably higher.

 

The autonomic tonus of the patients from the second and the third groups re-distributed by the end of the treatment. It occurred mainly due to the decrease of sympathetic ANS influence (hypersympathicotonia change into sympathicotonia or eutonia). There was no such redistribution in the patients from the control group.

 

The psychological state was analyzed before and after the treatment in all patients. All psychological weightness index components improved (anxiety, depression, self-control, health in general, emotional security, vital power). There was a 16% increase in the first group, 48% – in the second and 43% in the third one.

 

We also analyzed the dynamics of indices that influence the patients’ lives considerably (paramenia, dyspareunia, pains in the intermenstrual period, defecation disorders). The positive dynamics was more considerable in the groups where SCENAR-therapy was used as compared with the control group.

Conclusion. From the data given above, we may conclude that SCENAR therapy produces an optimizing influence upon the patients’ autonomic status (decreases the sympathetic ANS part influence and activates a parasympathetic ANS part), their psycho-emotional state, hemodynamic indices in the small pelvis organs, free-radical and general antioxidative activity, which improves homeostatic indices and quality of patient’s life as well as fertility restoration results.

SCENAR-therapy lets us improve the uterine tubes patency and rate of getting pregnant spontaneously. SCENAR-therapy results after the laparascopy, as well as without it, let us exclude laparascopy as a method of fallopian tubes correction provided that there are no hydrosalpinxes and that infertility period doesn’t exceed 3 years.

Evaluating SCENAR-therapy clinical effectiveness for the patients with tubal-peritoneal infertility lets us make a conclusion that it is an adequate method of rehabilitation treatment.

USING ELECTRIC PULSE THERAPY IN THE EARLY POSTOPERATIVE PERIOD AFTER SURGERY FOR TUBO-PERITONEAL INFERTILITY

USING ELECTRIC PULSE THERAPY IN THE EARLY POSTOPERATIVE PERIOD AFTER SURGERY FOR TUBO-PERITONEAL INFERTILITY

PublicationNon-drug medicine Journal, Issue No 3, 2010, pp.92-102

Authors:  Irina Holmogorova, MD, Candidate of Medical Sciences –  reflexotherapist, physical therapist, Physical Therapy Department of N.A. Semashko Nizhny Novgorod Regional Clinical Hospital, the State Health Organization

Key word: rehabilitation therapy for tubo-peritoneal infertility.

One of the current issues of restorative medicine in gynecology is the development of new effective non-drug therapies for tuboperitoneal infertility (TPI) – most common, well-researched, but most treatment-resistant type of infertility in women (V.M.Strugatskiy et al., 2001; A.N.Razumov et al., 2002; L.V.Dubnitskaya et al., 2006).

Rehabilitation treatment in early post-surgical period after tuboplasty is of high priority in solving this problem. Therefore alternative rehabilitation therapies need to be developed as the incidence of post-surgical complications is very high: recurrent adhesions, reocclusion and functional incompetence of fallopian tubes in 70-80% post-surgical patients (T.N.Revadenko, 1990, Yamada Y., 1990, M.A.Aliyev, 1998, G.A.Paladi, 1997, Carl M. Herbert, 2006 et.al).

In the early postoperative period after laparoscopic operations for TPI, rehabilitation therapy must solve some problems such as providing analgetic and anti-inflammatory effects, intensifying microcirculation processes, preventing the development of adhesive process in the small pelvis, maintenance of fallopian tube patency, faster rehabilitation, reducing emotional stress (O.V.Yarustovskaya, 2009, and others).

New pathogenetically substantiated methods that would simultaneously act on the driving mechanisms of pathogenesis, increase therapy effectiveness and result in less drug intake (A.N.Razumov, 2002-2008, T.E. Belousova, 2007 and others). On this basis, only that method which would improve the patient’s health as a whole can be considered effective (E.A.Turova, A.V.Golovach, 2005 and others).

SCENAR-therapy is scientifically interesting and prospective treatment modality in early postsurgical period after tuboplasty, which combines the principles of physio- and reflexotherapy (A.N.Razumov, I.P Bobrovnitskiy, A.M.Vasilenko, 2009).

Therefore, the objective of this research was to develop and provide scientific background for SCENAR-therapy in multiple rehabilitation treatment of patients after tuboplasty. 

Materials and methods. According to the objective and tasks set, 130 patients after laparoscopically assisted tuboplasty participated in the comprehensive clinical and laboratory examination and rehabilitation treatment. All the patients were of similar age (average age 28.4 + 1.7 years), with similar duration of the disease (in average 4.97 + 0.19 years), concomitant pathologies and clinical and functional data. Study groups included only women without any pathological changes in their endocrine profile. All the examined women were divided into 3 groups.

Group I (control group) included 30 patients who received only drug therapy in early post-operative period after laparoscopic surgery for TPI.

Group II (50 patients) – in early post-surgical period, starting from the 1st day,  patients received drug therapy and electric pulse therapy with the SCENAR-1-NT device following the combined method that included external abdominosacral method (10-15 minute stimulation); local vaginal method (15 minute stimulation); stimulation of acupuncture points GI 11, E36, RP 6, 2 minutes each using the subjectively dosed mode with 90Hz frequency. Total stimulation time was 35-40 minutes, 10 daily sessions during the treatment course.

Group III (50 patients) – patients who received drug therapy and electric pulse therapy with the SCENAR-1-NT device following the external method and stimulation of acupuncture points in early post-surgical period, starting from the 1st day. Stimulation settings and stimulation zones were the same as in group II.

All patients underwent general clinical and laboratory examination, ultrasound investigation of the bloodflow in the uterine and ovarian arteries using the Doppler sonography; analysis of general antioxidant activity of the blood plasma and intensity of free radical oxidation processes;  cardiointervalography  to evaluate patient’s atonomic status; questionnaire survey to evaluate patient’s atonomic status, the questionnaire was developed by the Russian Center for Autonomic Pathology; analysis of patient’s psychological state using the questionnaire survey to calculate the index of general psychological wellness (by G. Dupuis, 1984, D.A. Rivitsky, 1996).

2-3 months after the rehabilitation treatment all the patients had hysterosalpingography with water solutions of contrast agents following the standard practice.

The restoration of reproductive function was stated when the woman got spontaneously pregnant within one year. The male factor in women’s infertility was excluded basing on spermogram analysis and andrologist consultation

Results and discussion. The research established that in all groups the major complaints were weakness, lack of energy, indisposition, headaches, increase in the axillary temperature up to 37.5 °C, cramps, intestinal dysfunction. After the treatment, a regression of clinical symptoms in the group I was observed no sooner than by 13th-14th day of the postoperative period, and was characterized by normal body temperature (p=0.01), decreased pain (p=0.004). In the group II that followed combined methods of SCENAR-therapy a regression of clinical symptoms was observed significantly (p=0.003; p=0.001 respectively) earlier – by 8th-9th day. In group III, where SCENAR was applied externally, the regression of clinical symptoms was observed by 10th-11th day (p=0.005; p=0.002 respectively). This is most likely due to SCENAR capability to produce anti-inflammatory and analgetic effects.

An important aspect that provides the medical and economic significance is the reduction of in-hospital stay duration. For group I the duration of in-hospital stay of patients averaged 13.9 ± 1.92 days, for  those who followed the combined method (group II) the results significantly differed from those of the group I, duration of in-hospital stay was 8.7 ± 0.26 days, and for patients who were treated with SCENAR externally (group III) – 9.6 ± 1.13 days.

An important aspect that determines the clinical effectiveness of rehabilitation treatment is the fertility index that depends, particularly but not exclusively, on fallopian tube patency.

In group I, the percentage of tube patency preservation was 33% (10 patients), in group II – 74% (37 patients), in group III – 56% (28 patients) (see Fig.1).

Fig 1. Comparative analysis of treatment results for maintenance of fallopian tube patency

The results for maintenance of fallopian tube patency in groups II and III, are significantly better that those in group I (p=0.001, p=0.04). The assessment of treatment effectiveness as to such criterion as pregnancy occurrence showed that in group I this index was 23% (7 patients), in group II – 66% (33 patients), in group III – 46% (23 patients) (Fig.2).

 

Fig 2. Comparative analysis of treatment results for spontaneous pregnancy occurrence

The treatment provided a significantly better effect in groups II and III than in the group I (p=0.001, p=0.04). What is more, in group II where the combined method was used, it produced higher indices for maintenance of fallopian tube patency (p=0.04) and occurrence of spontaneous pregnancy (p=0.03), as compared with group III.

So, SCENAR-therapy in multiple drug therapy in early postsurgical period improves treatment effectiveness as a prophylaxis of reocclusion of uterine tubes and increases the generative function by 43%.

SCENAR-therapy in multiple rehabilitation treatment of patients after tuboplasty contributes to improvement of regional hemodynamics, which is greater when following the combined method, that is proved by the 22% increase of resistance index in the uterine arteries and 23% increase of the resistance index in the ovarian arteries.

SCENAR-therapy in treating patients after tuboplasty neutralizes negative changes in the state of lipid peroxidation system and antioxidant system, greatly decreases lipid peroxidation and increases antioxidant activity. At this the correction of lipid peroxidation and antioxidant system indices is significantly more often observed when following the combined method.

SCENAR-therapy in early postsurgical period after tuboplasty decreases the stress of body regulatory mechanisms, decreases the influence of sympathetic and increases the tone of parasympathetic systems, which is indicative of increased stability of autonomic regulation. SCENAR provides evident psychocorrective effect, manifested as decrease of anxiety level, depression, increase of self-control, general health and emotional well-being.

SCENAR-therapy in multiple rehabilitation treatment of patients in early postsurgical period after tuboplasty helps the patients to overcome the postsurgical period easier, decreases the intensity of pain syndrome, and promotes reduction of the in-hospital period. Combined method is by 32% more effective than the external one.

Summarizing all the above, we should emphasize that SCENAR-therapy is an effective treatment modality for rehabilitation of patients after tuboplasty in early postsurgical period.

The analysis of literature data and obtained results gives reason to believe that SCENAR-therapy action is provided by its beneficial effect on regional circulation. The improvement of regional hemodynamics, in turn, decreases the intensity of inflammations in the organs that are reflex-related to the area treated, and blocks pain receptors. The other effects are improved trophic functions of pelvic organs, which improves functional activity of fallopian tubes, prevents adhesions, and promotes higher percentage of preserved tube patency and occurrence of spontaneous pregnancy in the long-term period. A significantly better effect provided by combined method of SCENAR-therapy is most likely due to the mechanism of therapeutic action of vaginal physical treatment that, according to A.N.Obrosov (1965) and S.V.Vdovin (1980) are based on reflex response realized neurohumorally, and in J. Huberta’s (1985) opinion – the groups of vaginal acupuncture points make trigger zones that unite primary acupuncture channels – specifically, the stomach, spleen-pancrease and conception vessel meridians that can restore the reproductive function. The developed rehabilitation therapy that includes SCENAR-therapy as a component of multiple treatment in the early postoperative period after tuboplasty can be recommended for practice in the system of medical and prophylactic institutions.

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2010 Australasian RITMSCENAR™ Conference

2010 Australasian SCENAR™ Conference Details

The RITM SCENAR Institute – Australia , RITM Australia, and Scenar Health Australia are pleased to announce details of the 2010 Australasian SCENAR Conference.
Dates
July 30 – August 2 at Darling Harbour, Sydney.
Guest Lecturers
Prof. Alexander Tarakanov, M.D., Head of Acute Care and Emergency Department, Rostov State Medical University.
Dr Donese Worden NMD
• More to be announced.
Program
To be announced.
Venue
Novotel Rockford Darling Harbour
17 Little Pier Street
Darling Harbour NSW 2000
Prices
• AU$1450
AU$1350 – Early Bird discount – book and pay by May 15 and receive a $100 discount
AU$1350 – STAA members
Hotel room rates in Novotel Rockford Darling Harbour
Standard King room only is $179.00 to include a full breakfast buffet is an extra $15.00 per person
Please quote RITM SCENAR Institute Training to get the special rate.
Social program (included in conference price)
Sydney Princes Boat Cruise on 1st of August, 7pm – 10pm (drinks not included).
AU$80 for Accompanying person

Physical influencing factors in SCENAR-therapy

Physical influencing factors in SCENAR-therapy
Application sound therapy
Jakov Grinberg

Publication: Southern Federal University Bulletin. Technical
Science” Journal, Vol 99, Issue 10, 2009, pp. 123-128

The structure chart of SCENAR devices includes impulse oscillator, control unit, output cascade loaded on the high-quality and more often on the autotransformer circuit, passive and active electrodes. The structure allows to utilize the capabilities of effective electric impulse action based on use of appropriate signal shape, variation of pulse’s amplitude-frequency characteristics and some other tricks [1-7]. And still, this structural chart is similar to existing analogues. In such devices, only electric current is considered as an influencing physical factor.
Our study shows that therapeutic effect of SCENAR can be provided by several factors, and some of them take a new look at well-known treatments.
The objective of this research is to investigate and analyze physical influencing factors and represent SCENAR-therapy as a new class of electric and musical treatment, with a strong and pronounced vibrotactile (application) component.

Electric pulses

Electric pulses are the major influencing factor. Pulses can be represented as a damped sinusoid (Fig. 1). Upon putting the electrode on the skin, the damped oscillation frequency always decreases (pulse is extending), the oscillation number reduces as compared with that of the signal before the electrode is applied on the skin. At the same time, during therapy (after the electrode is put on the skin), pulses keep extending, and the oscillation number may decrease (which is more often the case) as well as increase. That depends on changes in capacitive and resistive components of the skin impedance (and their relation) between the skin and electrode. At a given power level, voltage amplitude decreases greatly, while current amplitude almost doesn’t change (Fig.2, 3). And SCENAR allows to get various modulations of the generated signal. It should also be considered that most stimulus energy is concentrated on the electrode-skin border.

 

Fig. 1. Electrode voltage waveform before the electrode is put on skin

Fig.2 Variants of voltage waveforms in SCENAR stimulation. 1 – signal in 0.22 sec (13th pulse); 2 – signal in 1 sec (60th pulse); 3 – signal in 10 sec (600th pulse).

Fig.3 Variants of current waveforms in SCENAR stimulation. 1 – signal in 0.22 sec (13th pulse); 2 – signal in 1 sec (60th pulse); 3 – signal in 10 sec (600th pulse).
High-frequency massage
High-frequency massage is inherently connected with electric pulses. High stimulation amplitude (Fig 1,2 represent actual amplitude values that makes 400-500 V at the moment of touching, and up to 200V in the mode of therapy) causes vibrations of the corneal layer (as well as lucid, if any) similar as flexible film in the electrostatic loudspeaker [8-13]. At this the skin sound can be listened. Pulse rate is 14-350Hz, but spectrums of stimulating sound and vibrations are too wide, i.e. frequency components are up to dozens of kHz and more. SCENAR devices provide various complex modulations of the signal, thus greatly enriching the spectrum with low and high frequency components (see below).
In physiotherapy (considering pulse length) signals (Fig. 1-3) can be regarded as low frequent [14]. In mechanical vibrotherapy low frequency is 20-50Hz, while high frequency is 100-200Hz. In the instant case we deal with short pulses of a wide spectrum, so we called it high-frequency massage.
It should be emphasized that vibration and sound are determined by the direct influence of high alternating electric field.

Influencing factors spectrum

The point of spectrum of the influencing factors is very important and demands greater attention to it. In SCENAR-therapy electric pulse (Fig. 1) is damped sinusoid described as u(t) =U e-?tsin?0t, where U – amplitude, ? – coefficient that characterizes how quickly the sinusoid damps, ?0 – damping sinusoid frequency.
Fig. 4 shows the spectral forms of the damped sinusoid (envelope), which greatly depend on ?. In moderate damping of the sinusoid (electric pulse before putting the electrode on the skin, Fig. 1) the spectrum has a pronounced maximum (for the pulse in the Fig. 1 with sinusoid period 16 microseconds it equals the frequency f = 62.5kHz).

Fig. 4
Spectrum of damping sinusoid pulse. With ? increase it becomes plain. The fragment shows the spectrum of repetition pulse train in the figure for 90Hz.
When the electrode is put on the skin ? value is too great and spectrum width is about 1.5-2 f0, where f0 _ vibration rate of the damping sinusoid. For example, for the pulse in 0.22sec (Fig. 2,3, signal 1) f0 = 18khz and spectrum width = 27kHz, while in 10sec (Fig. 2,3, signal 3) spectrum width is about 17kHz.
Repetition pulse train in SCENAR-stimulation is 15-350Hz. Spectrum of repetition pulse train includes harmonics of basic frequency, whose envelope is in form with the spectrum envelope of a single pulse. The fragment shows spectrum of repetition pulse train for the frequency f=90Hz (3 harmonic components 90, 180 and 270Hz).
It should be noted that the signal spectrum in SCENAR-therapy is pretty complicated. In the instant example the peak frequency of the electrode when touching the skin is 62.5Hz, in 22sec the spectrum moves to 18Hz, in 1 sec – to 15Hz, in 10sec – to 11Hz. The spectrum enriches with the slow components that are determined by pulse dynamics. Additional modulations make the spectrum more diverse and enriched. Thus the spectrum includes both the components determined by the pulse change rate and the components defined by the modulation as well as different combinations of these harmonics.

Mechanical action

In fact, high-frequency massage is also a factor of mechanical influence. Here we will consider the factor connected with different techniques. SCENAR-therapists often use labile stimulation technique in their work [13], when the electrode is moved along the skin as if massaging the skin in straight lines, circles, spirals, etc. It should be noted that the author of the techniques (Yu.V. Gorfinkel) used stabile and labile stimulation in his work when vibration component was unknown. At the same time such techniques are widely used in vibrotherapy [14].

Metal transfer

The problem of metal transfer is connected with high current density in SCENAR-therapy. This issue has never been investigated though many users pay attention that SCENAR electrode becomes pecky in case it was used too long.
The amplitude of first half-wave of electric current in SCENAR-therapy is about 10-100 milliampere (mA), depending on the patient’s individual sensitivity. So, considering that the electrode area is not so large (central part is about 2cm2) current density is about 5-50mA/cm2 and in, for example, sinusoidal modulated current therapy 50-500 times exceeds this value. That seems to be the reason for metal microtransfer.
Different metals are proven to have positive effect in treatment. Iron positively effects bone tissues, liver, spleen and blood. Gold tones up nervous system and myocard, improves memory and gives energy to the whole body. Silver helps in exhaustion, heartburn, chronic fever, and inflammatory bowel disease. Copper has a positive effect on liver, spleen and lymphatic system functionality. So treating patients with the electrode of a particular metal we may expect a secondary positive effect of SCENAR-therapy.

Impact on Intercellular Fluid

The problem of impact on intercellular fluid is connected with the high amplitude of electric pulses [12] and also requires additional investigations. Liquid environment of the body mainly consists of water, and water molecules are polar. When supplying the alternating voltage, the dipole is rotating according to the field change at a stimulation frequency and its harmonics. There are hypotheses that every human cell is surrounded by water molecules. If it’s a cell of a diseased organ, then, irrespective of disease, it is surrounded by so called non-structured water. A cell of a healthy organ is surrounded by structured water, and the body itself structures it at the cellular level. Hypothetically we can assert that SCENAR promotes structuring of water (fluid) around cells, and this provides a direction for recovery of the body [12].

Double-layer capacity formation

Formation of a double-layer capacity in the indirect factor that may influence SCENAR-therapy due to pulse modulation via skin-electrode interaction. Metal ions moving to the electrolytic solution and reverse movement of mobile ions from the liquid surrounding the electrode to its surface may also influence the given therapy. Moreover, dynamic properties of the signal, heavily influenced by the double-layer capacity formation, are used in SCENAR-techniques.
The formation of the double-layer capacity is connected with the phenomena occurring on the border of the first (electron) and second (ion) class conductors [8-10]. The metal is in contact with a complex of water solutions including a number of inorganic as well as organic electrolytes. The potential difference (double electric layer) that occurs on the metal-solution border is called the electrode potential.
The double layer is formed due to the movement of metal ions to the electrolytic solution and reverse motion of mobile ions from the liquid around the electrode to its surface. The formation lasts for about 0.5-1 sec.
Then, electrochemical reactions associated with the local metabolism develop between the electrode and solution. This provides the subsequent dynamics, slow changes of electrode potential, and thus, those of the double layer capacity.
On switching the device on, the effects of current pulses are imposed upon the picture described above. Signal variability (Fig.1 to 3) is determined by these two processes: forming of double layer capacity and effect of current pulses.
SCENAR-therapy features a great number of influencing physical factors, but identifying feature of SCENAR-devices is simultaneous action of high amplitude electric pulses and pulsating electric field, which causes high-frequency massage of subjacent tissues. Further on we will consider one of the possible aspects of such influence.

Application Music Therapy

Therapeutic effect of sound therapy is based on the influence of sounds and vibrations of human voice, musical instruments, natural powers and animal world.
Music therapy is a popular and growing medical field in which music and singing is used within a therapeutic relationship. Music therapist uses different treatment thechniques in each concrete case.
Already ancient documents and treatises mention therapeutic effect of the music (Pythagoras, Aristotle, Platon, Avizenna).
Musical stimulation influences CNS and functions of vital physiological systems. Melodies that make people happy are proven to slow down the pulse, increase heart forse, contribute to widening of blood vessels and normalisation of blood pressure, while the irritative music has absolutely opposite effect.
Music perception is roughly divided into acoustic and vibrotactile components.
Acoustic perception is realized via acoustic analyzer and allows to perceive acoustic signals of 20-20000Hz (acoustic analyzer enables/allows perceiving sound signals of 20-20000Hz), and vibrotactile component is considered to cause vibrations in the whole body. In music therapy acoustic waves are formed into a musical structure and are supposed to produce a simultaneous impact on the emotional and spiritual spheres of the human, as well as direct impact on the body surface and inner organs. The latter (direct influence on the body surface) is a part of alternative (bypassing organs of hearing, application) music therapy (it should be noted that according to a hypothesis nerve activity is based on transmitting musical vibrations, not electric pulses). Application music therapy is realized by influencing organ projections with acoustic signals. The instance of application therapy when sound-emitting device influenced kidney projection and microphones (loud speakers) influenced biologically active zones [15]. There are theoretical investigations and practical application of simultaneous electric musical influence [16]. The impact is realized by musical electric signal and its simultaneous listening, i.e. combination of electric musical therapy and “usual” (common in music therapy) vibrotactile component. Doctor chooses the composition, which gives positive emotions to a patient, and may also add R. Voll’s therapeutic frequencies superimposed on music. Further on we will show that SCENAR-therapy as a new class of electric and musical treatment, with a strong and pronounced vibrotactile (application) component.

Application action

It is worth mentioning again that the SCENAR effect lies in skin conversion of the stimulus into the sound, like in the electrostatic loudspeaker. That means that in terms of music therapy SCENAR-stimulation is application stimulation by nature. Specific feature of SCENAR-therapy is that vibrotactile (application) component of the stimulation is realized on the background of short pulses of high amplitude current. Two other major features are determined by the spectrum and vibrotactile component level.

Vibtotactile component spectrum

Spectrum of SCENAR stimulus is pretty complex and consists of portions of Hz (for example, in amplitude modulation 3:1 component harmonics is 0.25 Hz) to dozens of kHz. Vibtotactile component spectrum is determined by frequency characteristic of the voltage-to-sound conversion. In loudspeakers it is called sound pressure frequency characteristic. Sound pressure is the local pressure deviation from the average pressure caused by a sound wave. The results of several pilot studies (together with M.A. Unakafov) show that vibtotactile component spectrum is narrower than that of the signal, but still is too wide. When influencing factors are used in physiotherapy, low frequency vibration (up to 40Hz) and high frequency vibration (up to 250Hz) are distinguished. Stimulation spectrum in high-frequency massage is of pulse behavior and accounts for several kilohertz at least.

Vibrotactile component level

We have already mentioned that sound pressure is one of the characteristic features of the loudspeaker. Upon evaluating the vibrotactile (application) component level it seems quite reasonable to come to the term sound pressure – acoustic radiation pressure of the body in the stationary sound field. The acoustic radiation pressure is low if compared with the sound pressure (about three times below the sound pressure level). That means that vibrotactile component is too small in usual (pleasing) music composition. In SCENAR-therapy the skin itself (corneal layer) exerts sound pressure on the body. So, it can be stated for sure that the level of such stimulation is several times higher than the common (when listening to music) sound pressure level. That opens new prospects for using obtained theoretical and practical findings of music therapy.

Implemented procedures

SCENAR-devices realize three types of musical modulations that are similar to that in music therapy [13].
1. Patient-specific modulations that consider zodiacal sign. For example, C major key compositions are for Aries, while A major key is for Capricorn.
2. Set of keys (modulations) for the therapy in each particular case. Different articles and publications on music therapy state that Beethoven’s and Mozart’s compositions are indicated for people with gastrointestinal diseases; D-flat major compositions treat eye diseases and migraine; D major compositions help in treating kidneys and urinary bladder; rheumatism calms down when listening F major compositions.
3. Musical compositions that bring positive emotions added on user’s request.

Conclusion

1. SCENAR-therapeutic effect is provided by several influencing factors.
2. High-frequency massage, which can be considered as alternative (application) sound therapy, is one of the major secondary factors.
3. Application component in SCENAR-therapy is the action of wide spectrum and unique high stimulation level.
4. SCENAR-therapy with musical modulations is a new class of electric and musical treatment, with a strong and pronounced vibrotactile (application) component.

Pioneering development in stimulation of healing process

Pioneering development in stimulation of healing process

The article is in German Language

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Influence of SCENAR-therapy on the pregnancy course, labor, state of a neonate and a first-year child in women with miscarriage of infectious genesis

Publication: Russian reporter of Gynaecologists and Obstetricians 2, 2009
Authors: L.V. Borovkova, A.A. Artifeksova, S.O. Kolobova
Nizhny Novgorod State Medical Academy
Obstetrics and Gynecology Department
Head of the Department – L.V. Borovkova, Professor

Influence of SCENAR-therapy on the pregnancy course, labor, state of a neonate and a first-year child in women with miscarriage of infectious genesis

Abstract: We investigated effectiveness of SCENAR-therapy in complex treatment of 60 pregnant women with urogenital infections at 16–18 weeks of pregnancy. The control group included 50 pregnant women with similar urogenital infections that underwent conventional antibacterial and antiviral therapy after 16–18 weeks of pregnancy. Using SCENAR-therapy in treating pregnant women with miscarriage of infectious genesis significantly improves prognosis of bearing a child, the state of a fetus, neonate and a first-year child, decreased the incidence and severity of placental inflammatory processes and intrauterine infection.

Miscarriage is not only current medical concern, but also a serious social-economic problem with the case rate 15-20% among all desirable pregnancies. Today urogenital infection is one of the major causes of miscarriage, which may have harmful effect on the course of the pregnancy, state of a fetus and a neonate. Infectious pathology is mainly caused by genital microplasmas, clamydia, herpes simplex virus (HSV) and cytomegalovirus (CMV) [5, 7, 12].

New conditions for infection development are created during gestation. It is connected with general and local immunity peculiarities of pregnant women. Systemic gestational immunosuppression (suppression of inflammatory TH-1-immune response route) in healthy women provides formation of immune tolerance to semiallogenic fetus and pregnancy bearing. During development of acute urogenital infection or reactivation of chronic urogenital infection the T-helpers in the pregnant body significantly decrease and that activates local and general cytotoxic immune, as well as causes depression of humoral Th-2-immune response route. Infectious agents cause hypersynthesis of antiflammatory cytotoxicants (TNF, interleukin-1) providing prothrombinase overproduction, activation of coagulation mechanisms, trombosis in trophoblast vessels, destruction of vascular endothelial and trophoblast damage, as well as production of antiphospholipid and anti-DNA antibodies and formation of specific cytotoxic immune response directed against fetus antigens. Hyper amount of Th-1-route cytoxines increases the production of prostaglandin in amnion and decidua and may cause abortion [7, 11, 12, 14, 15].

It is proved that even massive virus and bacterial infections may not influence the course of pregnancy and fetus development in case a pregnant woman has no evident changes in the immunity. So, negative effect any infection has on a fetus and a neonate mainly depends on the intensity and character of immune status changes of a pregnant woman [12].

Infectious agents may cause certain complications in the course of the pregnancy: abortion (31.7-67.5%), early gestosis (32.4%), gestosis (23.7-49%), pyelonephritis (7-21.4%), anemia (92%), production of autoimmune antibodies (19-51.1%), fetoplacental insufficiency progression (47.6-73.3%), and preterm labor (8.8-16%). Women with urogenital infections may have the following complications during the childbirth: preterm amniorrhea (27.7-39%), pathological preliminary period (6.6-7.1%), labor anomaly (7.1-38.5%). In all types of infection afterbirth period is complicated by subtle late-onset (5-7 days after labor) endometritis (8.4-21.4%) with relapses and uterus subinvolution (13.5%) [5, 12].

It is known that placenta and embryonic membranes protect the fetus from bacteria and viruses. In most cases infectious process just affects placenta and starts the development of adaptative reactions in it (increase in villi vascularization, formation of syncytial node, villi proliferation with increase in placenta volume and mass). At this a newborn can be healthy with good physical and functional parameters. If the effect of infectious activator is too long or repeated, placental porosity excoriates causing the development of placental insufficiency and infectious process in the fetus. Literature says that when bacterial and virus infection inflammatory changes in placenta occur in 11-78.4% cases, placental insufficiency – 50-60%., intrauterine infection develops in 6-53% cases. High incidence rate of fetus infection was observed when inflammation of placenta fetus part and umbilical cord are combined, as well as in virus-virus or virus-bacterial associations. 36.8% of neonates born of women with placental infectious changes have infectious diseases, 25% – hypoxic syndrome and increase of the adaptation period, 55.7% – late cord remain rejection [8, 11, 12, 16, 17, 19].

Intrauterine infection by micoplasmas is observed in 35%, by ureaplasmas – 45%, manifestations of micoureaplasmic fetus infection are aspiration syndrome, interstitial pneumonia, cardiopathy. The most common infections among neonates are: meningitis, encephalitis, skin necrosis, conjunctivitis, pneumonia, osteomyelitis, hemorrhagic syndrome. Intrauterine infection by clamydia is observed in 9.7-63.3% and manifests in a generalized form (affection of eyes, kidneys, skin, lymph nodes), meningoencephalitis, gastroenteropathy, intrauterine pneumonia, conjunctivitis and vulvovaginitis in girls. Intrauterine infection in cytomegalovirus is observed in 0.2-40%, in herpetic infection – 0.01-75%. In most cases fetus infection is connected with primary virus infection during pregnancy. The fetus may be infected transplacental or while passing through the infected maternal passages. In cytomegalovirus the neonate can get infection through the infected breast milk. In cytomegalovirus the fetus has hydrocephaly, hydronephrosis, cystic changes in organs and tissues, as well as hepatitis, nephritis, encephalitis, myocarditis; neonates have the neonatal disease developing (low-birth-weight, jaundice, hepatosplenomegaly, pneumonia, meningoencephalitis, chorioretinitis, interstitial nephritis), developmental defects (microcephaly, paraventricular cyst, biliary atresia, multicystic kidneys, heart failure), late complications (deafness, blindness, encephalopathy, pneumosclerosis, cirrhosis, bowel and kidneys affection, etc). Herpes infection mainly affects the central nervous system, skin and mucous of a fetus, generalized form is also possible; congenital syndrome is observed in neonate (microcephaly, chorioretinitis, intracranial calcification) [5, 6, 9, 10, 12, 13].

If a fetus is infected but there are no manifestations of intrauterine infection, a neonate will have CNS (75-84%), CVS and GIT (26%) dysfunctions. Such children have high risk of developing infectious and allergic diseases, as well as psychomotor retardation during the first year of their life [12].

Modern complex antibacterial and immunocorrective therapy for pregnant women with urogenital infections doesn’t protect from intrauterine infection of the fetus, development of fetoplacental insufficiency, trombophilic complications, labor anomaly, as well as doesn’t improve perinatal outcomes. These are the reasons why doctors still search for new therapies for treating miscarriage of infectious genesis. In different publications we can find discussions on how urogenital infections influence the course of pregnancy and there are contrary points of view on whether pregnant women with urogenital infections should undergo immunocorrection or not. That is why we found it logical to study the effect of infections on the course of the pregnancy, labor, state of a neonate and 1-year child after pregnant women underwent traditional treatment, as well as complex therapy including SCENAR-therapy.

SCENAR is used for therapeutic non-invasive electropulse stimulation of the skin in different pathologies. Pulse bipolar alternate current is used as a stimulating signal sent as pulses with the frequency 10-350Hz including swinging frequency mode (30-120Hz) and can be combined into bursts (2-8 pulses) with repetition rate 540Hz-4.5kHz [18].

Previous researches of Nizhny Novgorod State Medical Academy [1-3] show that SCENAR-therapy has immunomodulatory and anti-inflammatory effect produced due to the decrease of anti-inflammatory citoxin levels. It also normalizes coagulate blood properties due to the decrease of thrombocyte ADP-aggregation and fibrinogen content, as well as antibody levels to cardiolipins, chronic gonadotropin and DNA.

The experiment with white mongrel rats showed that SCENAR short-pulse signals lack embriotoxic and teratogenic properties [4], and that allows to use SCENAR-therapy for treating pregnant women.

Using SCENAR-therapy for treating pregnant women is allowed upon authorization of local ethic committee under State Health Care Institution Nizniy Novgorod Regional Clinical Hospital named after N.A. Semashko. All the women gave a written consent for undergoing SCENAR-therapy.

Research aim was to compare the course of pregnancy, labor process, the state of a neonate and a first-year child of women with miscarriage of infectious genesis while treating urogenital infections with traditional therapies, as well as complex therapy including SCENAR-treatment.

Materials and methods

110 women with urogenital infections composed a control group. All the women were examined, observed clinically during pregnancy, as well as during the childbirth. We also examined the state of the fetus and a neonate.

All the patients were examined at 10-12, 22-24, 36-38 weeks of pregnancy for any manifestations of urogenital infections (clamidiosis, mycoplasmosis, ureaplasmosis, cytomegalovirus and herpetic infections). To diagnose micoplasmosis and ureaplasmosis we used bacteriological test – inoculation of cervical canal. To find clamidiosis, cytomegalovirus and herpetic infections we examined separate cervical canal using the method of polymerase chain reaction (PCR) and blood serum for antibodies (immunoglobulin class A, M, G) to the abovementioned infections using enzyme immunoassay.

In the postnatal period we studied morphological picture of 30 placentas, all the neonates underwent saliva and tear PCR-diagnostics, as well as cord blood EIA to find urogenital infections. First-year children were followed up by neuropathologist.

All the women we observed had urogenital infections as monoinfections (34.5%) or bacterial-virus associations (65.5%). Patients were divided into 2 groups considering their age, social status, past gynecological and extragenital diseases. Patients from the control group (50 women) underwent traditional antibacterial and antivirus therapy after 16-18 weeks of pregnancy. While patients from the main group (60 women) after 16-18 weeks of pregnancy underwent complex therapy with SCENAR-stimulation for 10 days according to the common method, including stimulation of cervical area, vertebral und paravertebral lines, 6 points, liver projection area.

The results were processed using application package for static Excel data processing and accurate difference between the indices was estimated according to the Student’s test. Difference between the compared values were considered as accurate when p<0.05.

Research results and discussion

When comparing clinical picture of the pregnancy we got accurate difference between the indices in main and control groups. So, in control group threatening miscarriage in the I and II pregnancy stages and threatened premature labor were registered in 26%, fetoplacental insufficiency – 20%, arrested fetus prenatal development of the I stage – 8%, dysfunction of utero-placental blood flow of the I stage – 10%. While in the main group miscarriage and fetoplacental insufficiency reduced twice. Acute infections (mainly micoplasmic-herpic and chlamidial-micoplasmic-cytomegalovirus) were observed in 31 (62%) women from the control group, where 10 (20%) had recurrent infection at 36-38 weeks, and in main group process reactivation was observed only in 11 (18.3%) cases and only at 20-24 weeks of pregnancy.

Accurate difference between the indices of the control groups was also manifested while analyzing the labor process. In the control group 44 (88%) women had spontaneous labor, 39 (78%) had labor in time, 5 (10%) – premature labor, 6 (12%) – cesarean section at 38-40 weeks. In the main group 55 (91.7%) women had labor in time and 5 (8.3%) – cesarean section at 38-40 weeks. In the control group 80% had xerotocia, compared to 60% in the main group, primary uterine inertia was observed in 30% and 20%, oxytocia – in 10% and 6.7% respectively. No complications observed in the afterbirth period in both groups. Clinical picture complications of the pregnancy and labor in women with miscarriage of infectious genesis are shown in the table.

The results presented in the table show that complications in the course of pregnancy and labor in women with miscarriage of infectious genesis in both (main and control) groups were observed mainly in the mixed bacterial-virus infection. Estimating the labor outcome for the fetus and the course of neonatal period we found out that in the control group neonates weighted 3206.6±154.7g, while in the main group the weight was 3280.6±176.4g. 5 neonates from the control group (10%) were born premature, 34 (68%) were born with a satisfactory state (8-10 Apgar scores), 9 (18%) – with light asphyxia neonatorum, 7 (14%) – with moderate asphyxia neonatorum and I degree hypertrophy, 16 (32%) – with adaptation disorders in the early neonatal period. In the main group the state of the neonates was much better: the state of 52 (86.7%) was estimated as 8-10 Apgar scores and only 8 (13.3%) had light asphyxia neonatorum and signs of adaptation disorders in the early neonatal period.

Complications in the course of pregnancy and labor Control group Main group
monoinfections bacterial-virus associations monoinfections bacterial-virus associations
Threatened miscarriage 3 10 2 5
Intrauterine growth retardation 0 4 0 0
Dysfunction of utero-placental blood flow of the I stage 0 5 0 0
Placenta premature ripening 0 4 0 4
Oligoamnios 0 2 0 3
Hydramnios 0 2 1 1
Preterm amniorrhea 13 27 10 26
Uterine inertia 5 10 3 9
Oxytocia 0 5 0 4
Preterm labor 0 5 0 0

Table. Complications in the course of pregnancy and labor in women with miscarriage of infectious genesis

Examination of placenta showed some peculiarities in its morphological structure, which depend on the type of urogenital infection and its therapies. Placenta histodiagnosis in the women from the main group showed no inflammatory changes in 9 (60%) cases (women with isolated micoplasmic or ureaplasmic, micoplasmic-ureoplasmic, chlamydial, chlamydial-herpic, micoplasmic-herpic infection). Only in 4 (26.7%) placentas (in chlamydial-cytomegaloviral, chlamydial-micoplasmic infection) we found out few small inflammation focuses in fetal membranes that localized within compact layer and cytotrophoblast, not reaching amniotic epithelium, and in basal membrane. In 2 (13.3%) cases (in women with chlamydial-cytomegaloviral, chlamydial-micoplasmic-cytomegaloviral infection) we manifested single small inflammatory infiltrates in fetal membranes including amniotic epithelium layer and subchorial space and in basal membrane.

Unfortunately only 2 (13.3%) patients (treated for ureaplasmos) from the control group had no inflammatory processes. 1 (6.7%) patient had single small inflammatory focuses in fetal membranes without any damages of amniotic epithelium and in basal membrane (observed after treating isolated micoplasmic infection). 2 (13.3%) patients had changes as small inflammatory infiltrates in fetal membranes, subchorial space and basal membrane. Most often these changes were observed after treating micoplasmic (ureaplasmic) infection. Examination of 10 (66.7%) placentas showed extensive inflammatory changes of the basal membrane, villi, intervillous lacuna, fetal membranes and subchorial space. 7 (46.7%) women out of them (mainly with chlamidial, herpic, chlamidial-herpic infections) had signs of relative compensated chronic placental insufficiency (according to the morphological diagnosis criteria of Tsinzerling and co-authors, 1998) with ill-defined focal involutive-dystrophic processes (focal dystrophic changes in syncytiotrophoblast, fibrinoid accumulation in intervillous lacuna) and well-marked adaptive reactions (hyperplasia and hypervascularization of terminal villi, enlargement of cyncytial nodes and syncytiocapillary membranes). In 3 (20%) women (with chlamydial-cytomegaloviral, chlamydial-micoplasmic-cytomegaloviral, chlamydial-micoplasmic infection) we observed signs of relative subcompensated chronic placental insufficiency with focal disorders in villi maturation (presence of embryonal, mature and immarure intervilli), diffusive fibrinoid accumulation, focal bleeding in intervillous space and active adaptive processes. So, in the main group inflammatory changes in the placentas developed after treating mixed bacterial-viral associations, had focal character and were observed 2.2 times less frequently compared to the control group, where inflammatory processes were well-marked, extensive and developed after treating both isolated and mixed infections. Moreover, the results showed that the most evident and well-marked changes in both groups were observed in mixed bacterial-virus infections.

Diagnosing bacterial and virus infections of the neonates showed that 34 (68%) in the control group) and 52 (86.7%) in the main group) children born in women both with isolated and mixed bacterial-virus infections had no bacterial and virus agents in their saliva and tear. Their blood serum contained specific IgG antibodies against infectious agents in the titer equal or twice smaller than the titer of corresponding mother antibodies. Literature says that signs not fetal infection, but placental antibody transmission [12, 20]. No specific IgM registered.

Analyzing the research results we found out that 16 (32%) neonates from the control group probably had fetal infection: in 9 (8%) neonates born in women that had acute chlamydial-cytomegaloviral, chlamydial-micoplasmic, micoplasmic-herpic infection at 20-24 week of pregnancy, IgG titer against chlamidiae, cytomegalovirus and hepric virus was twice higher than level of mother antibodies (that can sign fetal infection). No IgM found. Examining saliva and tear showed no bacterial and virus agents. Blood serum of 7 (14%) neonates, born in women that had reactivation of chlamydial-micoplasmic, chlamydial-micoplasmic-cytomegaloviral infections at 20-34 weeks and 36-38 weeks of pregnancy, contained high levels of specific IgM antibodies against chlamidiae and cytomegalovirus, titer of IgG against the infections mentioned was 2-4 times higher than the titer of mother antibodies. Examining saliva and tear showed no bacterial and virus agents.

In the main group signs of fetal infection were observed only in 8 (13.3%) neonates born in women that had acute chlamydial-micoplasmic-cytomegaloviral, micoplasmic-herpetic infection at 20-24 weeks of pregnancy. The titer of IgG antibodies against cytomegalovirus and simple herpes in these neonates was twice higher than the level of mother antibodies (that can sign fetal infection). IgG antibodies against chlamidiae was twice lower than titer of mother antibodies. No IgM found. Examining saliva and tear showed no bacterial and virus agents.

Observing the neuropsychic state of the 1-year child we found out that 34 (68%) children from the control group had normal psychomotor development. At this 16 (32%) neonates with light or moderate asphyxia born of women with chlamydial-micoplasmic-cytomegaloviral, micoplasmic-herpetic, chlamydial-micoplasmic, chlamydial-cytomegaloviral infections, extensive inflammatory changes of the fetus with signs of placental inefficiency, were observed at neurologist on perinatal encephalopathy and hypertension jitteriness (10, or 20% cases) and with psychomotor and preverbal retardation (6 or 12% cases). After they underwent treatment manifestations of perinatal encephalopathy by the end of the 1-year disappeared in 9 (8%) and remained in 7 (14%) children, whose mothers suffered from chlamydial-micoplasmic, chlamydial-micoplasmic-cytomegaloviral infections and had extensive inflammatory processes in placenta with signs of subcompensated placental inefficiency. In the main group 52 (86.7%) children had no neuropsychic disorders and only 8 (13.3%) neonates with light asphyxia born of women with chlamydial-micoplasmic-cytomegaloviral, micoplasmic-herpetic infections with ill-defined inflammatory changes of the placenta as focal chorioamnionitis and basal deciduitis had suffered from perinatal encephalopathy and hypertension jitteriness, treating which helped to relieve the symptoms and remove them by the end of the 1 year. So, in the main group the development of perinatal encephalopathy in 1-year children in mixed bacterial-virus infection of the mother was observed 2.4 times less frequently than in the control group. Moreover, in the main group by the end of the first year this pathology had been almost cured (compared to the control group).

Conclusions
1. Most often threatened miscarriage, development of fetoplacental insufficiency, labor anomaly, afterbirth pathology, intrauterine infection, neuropsychological abnormalities in the 1-year children were observed in patients with mixed bacterial-virus associations (compared to monoinfections).

2. SCENAR-therapy in pregnant women with miscarriage of infectious genesis significantly contributed to decrease the case rate and severity level of inflammatory processes in the placenta (from 86.7% cases with well-marked changes in the control group to 40% with ill-defined signs of infection in the main group).

3. SCENAR-therapy in treating pregnant women with miscarriage of infectious genesis significantly improves the prognosis of bearing a child, the state of a fetus, neonate, and first-year child, decreased the incidence of intrauterine infection neuropsychological abnormalities in the 1-year children.

References:

1. L. V. Borovkova. Reproductive function of patients with genital endometriosis: abstract of a thesis. M 2004; 51
2. L.V. Borovkova, I. E. Kholmogorova, V. D. Uchaikina and co-authors. Scientific substantiation of pulse therapy effectiveness for treating infertility in patients with external endometriosis. Reflexology 2005; 3: 58-60

Effect of Artrofoon and SCENAR Therapy on Parameters of LPO and Antioxidant System of the Blood in Patients with Peritonitis in Postoperative Period

Effect of Artrofoon and SCENAR Therapy on Parameters of LPO and Antioxidant System of the Blood in Patients with Peritonitis in Postoperative Period

A. V. Tarakanov, S. Kh. Luspikayan,
N. P. Milyutina, and A. V. Rozhkov

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 148, Suppl. 1, pp. 136-139, September, 2009
Original article submitted August 1, 2008

Administration of artrofoon in combination with SCENAR therapy to patients with localized suppurative peritonitis in the postoperative period considerably reduced plasma MDA level, stabilized ceruloplasmin activity, and increased catalase activity in erythrocytes compared to the corresponding parameters in patients receiving standard treatment in combination with SCENAR therapy.

Key Words: suppurative appendicular peritonitis; artrofoon; self-controllable energoneuroadaptive regulator (SCENAR); lipid peroxidation; antioxidant enzymes

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